human tnbc cell lines hcc38 Search Results


sample collection human breast cancer cell lines  (ATCC)
96
ATCC sample collection human breast cancer cell lines
Sample Collection Human Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brca1 deficient bc cells lines  (ATCC)
97
ATCC brca1 deficient bc cells lines
Brca1 Deficient Bc Cells Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tnbc cell lines  (ATCC)
99
ATCC tnbc cell lines
circKIF4A acts as a sponge for miR-375 a The expression levels of nuclear control (18S), cytoplasmic control (GAPDH) and circKIF4A were detected. b The predicted binding sites of miR-375 within the circKIF4A sequence. c The miR-375 expression in <t>TNBC</t> <t>cell</t> lines. d Luciferase assay of cells cotransfected with miR-375 mimics and wild type or mutant luciferase reporter. e MS2-based RIP assay transfected with MS2bs-circKIF4A, MS2bs-circKIF4Amt or control. f A CCK-8 assay to detect cell proliferation. g A colony formation assay to detect cell colony-forming ability. h The colony formation number was quantified by ImageJ. i A Transwell assay to assess cell migration ability. j The number of invasive was quantified by ImageJ. k A wound-healing assay to detect cell migration ability. l Wound closure was quantified by ImageJ. m-n Immunofluorescence staining for E-cadherin and vimentin. o Xenograft models were established. p Summary of the tumor weights. q Representative image of luciferase signals of lung metastatic nodules. r Representative images of lung metastatic nodules and HE-stained sections. s The number of metastatic nodules was quantified. ** P < 0.01
Tnbc Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human tnbc  (ATCC)
97
ATCC human tnbc
circKIF4A acts as a sponge for miR-375 a The expression levels of nuclear control (18S), cytoplasmic control (GAPDH) and circKIF4A were detected. b The predicted binding sites of miR-375 within the circKIF4A sequence. c The miR-375 expression in <t>TNBC</t> <t>cell</t> lines. d Luciferase assay of cells cotransfected with miR-375 mimics and wild type or mutant luciferase reporter. e MS2-based RIP assay transfected with MS2bs-circKIF4A, MS2bs-circKIF4Amt or control. f A CCK-8 assay to detect cell proliferation. g A colony formation assay to detect cell colony-forming ability. h The colony formation number was quantified by ImageJ. i A Transwell assay to assess cell migration ability. j The number of invasive was quantified by ImageJ. k A wound-healing assay to detect cell migration ability. l Wound closure was quantified by ImageJ. m-n Immunofluorescence staining for E-cadherin and vimentin. o Xenograft models were established. p Summary of the tumor weights. q Representative image of luciferase signals of lung metastatic nodules. r Representative images of lung metastatic nodules and HE-stained sections. s The number of metastatic nodules was quantified. ** P < 0.01
Human Tnbc, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human breast cancer cells  (ATCC)
97
ATCC human breast cancer cells
circKIF4A acts as a sponge for miR-375 a The expression levels of nuclear control (18S), cytoplasmic control (GAPDH) and circKIF4A were detected. b The predicted binding sites of miR-375 within the circKIF4A sequence. c The miR-375 expression in <t>TNBC</t> <t>cell</t> lines. d Luciferase assay of cells cotransfected with miR-375 mimics and wild type or mutant luciferase reporter. e MS2-based RIP assay transfected with MS2bs-circKIF4A, MS2bs-circKIF4Amt or control. f A CCK-8 assay to detect cell proliferation. g A colony formation assay to detect cell colony-forming ability. h The colony formation number was quantified by ImageJ. i A Transwell assay to assess cell migration ability. j The number of invasive was quantified by ImageJ. k A wound-healing assay to detect cell migration ability. l Wound closure was quantified by ImageJ. m-n Immunofluorescence staining for E-cadherin and vimentin. o Xenograft models were established. p Summary of the tumor weights. q Representative image of luciferase signals of lung metastatic nodules. r Representative images of lung metastatic nodules and HE-stained sections. s The number of metastatic nodules was quantified. ** P < 0.01
Human Breast Cancer Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human breast cancer cell lines  (ATCC)
99
ATCC human breast cancer cell lines
circKIF4A acts as a sponge for miR-375 a The expression levels of nuclear control (18S), cytoplasmic control (GAPDH) and circKIF4A were detected. b The predicted binding sites of miR-375 within the circKIF4A sequence. c The miR-375 expression in <t>TNBC</t> <t>cell</t> lines. d Luciferase assay of cells cotransfected with miR-375 mimics and wild type or mutant luciferase reporter. e MS2-based RIP assay transfected with MS2bs-circKIF4A, MS2bs-circKIF4Amt or control. f A CCK-8 assay to detect cell proliferation. g A colony formation assay to detect cell colony-forming ability. h The colony formation number was quantified by ImageJ. i A Transwell assay to assess cell migration ability. j The number of invasive was quantified by ImageJ. k A wound-healing assay to detect cell migration ability. l Wound closure was quantified by ImageJ. m-n Immunofluorescence staining for E-cadherin and vimentin. o Xenograft models were established. p Summary of the tumor weights. q Representative image of luciferase signals of lung metastatic nodules. r Representative images of lung metastatic nodules and HE-stained sections. s The number of metastatic nodules was quantified. ** P < 0.01
Human Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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brca cell lines  (ATCC)
97
ATCC brca cell lines
circKIF4A acts as a sponge for miR-375 a The expression levels of nuclear control (18S), cytoplasmic control (GAPDH) and circKIF4A were detected. b The predicted binding sites of miR-375 within the circKIF4A sequence. c The miR-375 expression in <t>TNBC</t> <t>cell</t> lines. d Luciferase assay of cells cotransfected with miR-375 mimics and wild type or mutant luciferase reporter. e MS2-based RIP assay transfected with MS2bs-circKIF4A, MS2bs-circKIF4Amt or control. f A CCK-8 assay to detect cell proliferation. g A colony formation assay to detect cell colony-forming ability. h The colony formation number was quantified by ImageJ. i A Transwell assay to assess cell migration ability. j The number of invasive was quantified by ImageJ. k A wound-healing assay to detect cell migration ability. l Wound closure was quantified by ImageJ. m-n Immunofluorescence staining for E-cadherin and vimentin. o Xenograft models were established. p Summary of the tumor weights. q Representative image of luciferase signals of lung metastatic nodules. r Representative images of lung metastatic nodules and HE-stained sections. s The number of metastatic nodules was quantified. ** P < 0.01
Brca Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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breast cancer cell lines  (ATCC)
96
ATCC breast cancer cell lines
circKIF4A acts as a sponge for miR-375 a The expression levels of nuclear control (18S), cytoplasmic control (GAPDH) and circKIF4A were detected. b The predicted binding sites of miR-375 within the circKIF4A sequence. c The miR-375 expression in <t>TNBC</t> <t>cell</t> lines. d Luciferase assay of cells cotransfected with miR-375 mimics and wild type or mutant luciferase reporter. e MS2-based RIP assay transfected with MS2bs-circKIF4A, MS2bs-circKIF4Amt or control. f A CCK-8 assay to detect cell proliferation. g A colony formation assay to detect cell colony-forming ability. h The colony formation number was quantified by ImageJ. i A Transwell assay to assess cell migration ability. j The number of invasive was quantified by ImageJ. k A wound-healing assay to detect cell migration ability. l Wound closure was quantified by ImageJ. m-n Immunofluorescence staining for E-cadherin and vimentin. o Xenograft models were established. p Summary of the tumor weights. q Representative image of luciferase signals of lung metastatic nodules. r Representative images of lung metastatic nodules and HE-stained sections. s The number of metastatic nodules was quantified. ** P < 0.01
Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary tumor specimens thirty breast cancer cell lines  (ATCC)
99
ATCC primary tumor specimens thirty breast cancer cell lines
circKIF4A acts as a sponge for miR-375 a The expression levels of nuclear control (18S), cytoplasmic control (GAPDH) and circKIF4A were detected. b The predicted binding sites of miR-375 within the circKIF4A sequence. c The miR-375 expression in <t>TNBC</t> <t>cell</t> lines. d Luciferase assay of cells cotransfected with miR-375 mimics and wild type or mutant luciferase reporter. e MS2-based RIP assay transfected with MS2bs-circKIF4A, MS2bs-circKIF4Amt or control. f A CCK-8 assay to detect cell proliferation. g A colony formation assay to detect cell colony-forming ability. h The colony formation number was quantified by ImageJ. i A Transwell assay to assess cell migration ability. j The number of invasive was quantified by ImageJ. k A wound-healing assay to detect cell migration ability. l Wound closure was quantified by ImageJ. m-n Immunofluorescence staining for E-cadherin and vimentin. o Xenograft models were established. p Summary of the tumor weights. q Representative image of luciferase signals of lung metastatic nodules. r Representative images of lung metastatic nodules and HE-stained sections. s The number of metastatic nodules was quantified. ** P < 0.01
Primary Tumor Specimens Thirty Breast Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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breast hcc38  (ATCC)
96
ATCC breast hcc38
circKIF4A acts as a sponge for miR-375 a The expression levels of nuclear control (18S), cytoplasmic control (GAPDH) and circKIF4A were detected. b The predicted binding sites of miR-375 within the circKIF4A sequence. c The miR-375 expression in <t>TNBC</t> <t>cell</t> lines. d Luciferase assay of cells cotransfected with miR-375 mimics and wild type or mutant luciferase reporter. e MS2-based RIP assay transfected with MS2bs-circKIF4A, MS2bs-circKIF4Amt or control. f A CCK-8 assay to detect cell proliferation. g A colony formation assay to detect cell colony-forming ability. h The colony formation number was quantified by ImageJ. i A Transwell assay to assess cell migration ability. j The number of invasive was quantified by ImageJ. k A wound-healing assay to detect cell migration ability. l Wound closure was quantified by ImageJ. m-n Immunofluorescence staining for E-cadherin and vimentin. o Xenograft models were established. p Summary of the tumor weights. q Representative image of luciferase signals of lung metastatic nodules. r Representative images of lung metastatic nodules and HE-stained sections. s The number of metastatic nodules was quantified. ** P < 0.01
Breast Hcc38, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human breast cell lines hcc38  (ATCC)
93
ATCC human breast cell lines hcc38
circKIF4A acts as a sponge for miR-375 a The expression levels of nuclear control (18S), cytoplasmic control (GAPDH) and circKIF4A were detected. b The predicted binding sites of miR-375 within the circKIF4A sequence. c The miR-375 expression in <t>TNBC</t> <t>cell</t> lines. d Luciferase assay of cells cotransfected with miR-375 mimics and wild type or mutant luciferase reporter. e MS2-based RIP assay transfected with MS2bs-circKIF4A, MS2bs-circKIF4Amt or control. f A CCK-8 assay to detect cell proliferation. g A colony formation assay to detect cell colony-forming ability. h The colony formation number was quantified by ImageJ. i A Transwell assay to assess cell migration ability. j The number of invasive was quantified by ImageJ. k A wound-healing assay to detect cell migration ability. l Wound closure was quantified by ImageJ. m-n Immunofluorescence staining for E-cadherin and vimentin. o Xenograft models were established. p Summary of the tumor weights. q Representative image of luciferase signals of lung metastatic nodules. r Representative images of lung metastatic nodules and HE-stained sections. s The number of metastatic nodules was quantified. ** P < 0.01
Human Breast Cell Lines Hcc38, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


circKIF4A acts as a sponge for miR-375 a The expression levels of nuclear control (18S), cytoplasmic control (GAPDH) and circKIF4A were detected. b The predicted binding sites of miR-375 within the circKIF4A sequence. c The miR-375 expression in TNBC cell lines. d Luciferase assay of cells cotransfected with miR-375 mimics and wild type or mutant luciferase reporter. e MS2-based RIP assay transfected with MS2bs-circKIF4A, MS2bs-circKIF4Amt or control. f A CCK-8 assay to detect cell proliferation. g A colony formation assay to detect cell colony-forming ability. h The colony formation number was quantified by ImageJ. i A Transwell assay to assess cell migration ability. j The number of invasive was quantified by ImageJ. k A wound-healing assay to detect cell migration ability. l Wound closure was quantified by ImageJ. m-n Immunofluorescence staining for E-cadherin and vimentin. o Xenograft models were established. p Summary of the tumor weights. q Representative image of luciferase signals of lung metastatic nodules. r Representative images of lung metastatic nodules and HE-stained sections. s The number of metastatic nodules was quantified. ** P < 0.01

Journal: Molecular Cancer

Article Title: circKIF4A acts as a prognostic factor and mediator to regulate the progression of triple-negative breast cancer

doi: 10.1186/s12943-019-0946-x

Figure Lengend Snippet: circKIF4A acts as a sponge for miR-375 a The expression levels of nuclear control (18S), cytoplasmic control (GAPDH) and circKIF4A were detected. b The predicted binding sites of miR-375 within the circKIF4A sequence. c The miR-375 expression in TNBC cell lines. d Luciferase assay of cells cotransfected with miR-375 mimics and wild type or mutant luciferase reporter. e MS2-based RIP assay transfected with MS2bs-circKIF4A, MS2bs-circKIF4Amt or control. f A CCK-8 assay to detect cell proliferation. g A colony formation assay to detect cell colony-forming ability. h The colony formation number was quantified by ImageJ. i A Transwell assay to assess cell migration ability. j The number of invasive was quantified by ImageJ. k A wound-healing assay to detect cell migration ability. l Wound closure was quantified by ImageJ. m-n Immunofluorescence staining for E-cadherin and vimentin. o Xenograft models were established. p Summary of the tumor weights. q Representative image of luciferase signals of lung metastatic nodules. r Representative images of lung metastatic nodules and HE-stained sections. s The number of metastatic nodules was quantified. ** P < 0.01

Article Snippet: All the cell lines, including a human mammary epithelial cell line (MCF10A), NTNBC cell lines (MCF-7, T47D, BT474 and SKBR3) and TNBC cell lines (MDA-MB-453, MDA-MB-468, MDA-MB-231, BT549 and HCC38), were purchased from American Type Culture Collection.

Techniques: Expressing, Control, Binding Assay, Sequencing, Luciferase, Mutagenesis, Transfection, CCK-8 Assay, Colony Assay, Transwell Assay, Migration, Wound Healing Assay, Immunofluorescence, Staining

circKIF4A acts as a ceRNA to regulate KIF4A a The predicted binding sites of miR-375 within the KIF4A 3’UTR. b Cells were transfected and luciferase assay was performed. c Cells were transfected and KIF4A expression was detected by qRT-PCR. d The KIF4A expression was detected by western blot (left) and quantified (right). e RIP assay showing the enrichment of circKIF4A, KIF4A and miR-375 on Ago2. f Cells were transfected and a RIP assay on Ago2 was performed. g Cells were transfected and KIF4A expression was detected by qRT-PCR. h KIF4A expression was detected by western blot (left) and quantified (right). i The KIF4A expression in TNBC cell lines was detected by qRT-PCR. j KIF4A expression in TNBC cell lines was determined by western blot (left) and quantified (right). k KIF4A expression in 57 pairs of TNBC tissues and normal adjacent tissues. l OS curves for 240 TNBC patients. m DFS curves for 240 TNBC patients. ** P < 0.01

Journal: Molecular Cancer

Article Title: circKIF4A acts as a prognostic factor and mediator to regulate the progression of triple-negative breast cancer

doi: 10.1186/s12943-019-0946-x

Figure Lengend Snippet: circKIF4A acts as a ceRNA to regulate KIF4A a The predicted binding sites of miR-375 within the KIF4A 3’UTR. b Cells were transfected and luciferase assay was performed. c Cells were transfected and KIF4A expression was detected by qRT-PCR. d The KIF4A expression was detected by western blot (left) and quantified (right). e RIP assay showing the enrichment of circKIF4A, KIF4A and miR-375 on Ago2. f Cells were transfected and a RIP assay on Ago2 was performed. g Cells were transfected and KIF4A expression was detected by qRT-PCR. h KIF4A expression was detected by western blot (left) and quantified (right). i The KIF4A expression in TNBC cell lines was detected by qRT-PCR. j KIF4A expression in TNBC cell lines was determined by western blot (left) and quantified (right). k KIF4A expression in 57 pairs of TNBC tissues and normal adjacent tissues. l OS curves for 240 TNBC patients. m DFS curves for 240 TNBC patients. ** P < 0.01

Article Snippet: All the cell lines, including a human mammary epithelial cell line (MCF10A), NTNBC cell lines (MCF-7, T47D, BT474 and SKBR3) and TNBC cell lines (MDA-MB-453, MDA-MB-468, MDA-MB-231, BT549 and HCC38), were purchased from American Type Culture Collection.

Techniques: Binding Assay, Transfection, Luciferase, Expressing, Quantitative RT-PCR, Western Blot